Biofertilizer based on halotolerant microorganisms promotes the growth of rice plants and alleviates the effects of saline stress.

Long-term soil salinization easily contributes to soil hardness, soil nutrient imbalance, and soil microbial diversity reduction, resulting in low rice yields in the salinized fields, and microbial remediation is one of the important measures to improve salinized soil. To verify the effect of biofertilizer based on halotolerant microorganisms on promoting rice growth and alleviating saline stress, this study discussed the effects of biofertilizer on soil microbial diversity and community structure and analyzed the correlation between the formation of microbial community structure and soil nutrient factors in the salinized field. The result, in comparison with applying inorganic fertilizer (referred to as CK), showed that notably increased soil available nitrogen, available phosphorus, available potassium, and rice paddy yield (p < 0.05) and significantly decreased soil electrical conductivity (p < 0.05) were achieved via biofertilizer (referred to as G2). Additionally, the application of biofertilizer contributes to the increase in soil microbial diversity and reorganization of microbial community structure, and through the analysis of linear discriminant analysis effect size, a notable difference in relative abundance was found in 13 genera, 6 families, and 3 orders between the control group and experimental groups (p < 0.05), and by linear discriminant analysis, Desulfomonas was further identified as the differentiated indicator. The redundancy analysis showed that available phosphorus and cation exchange capacity were the key environmental factors that affected microbial community structure and composition. Through bacterial functional prediction, increased rhizosphere soil bacterial metabolism, enzyme activity, membrane transport, and other potential functions were achieved by applying biofertilizer. Therefore, the application of biofertilizer could significantly alleviate rice growth stress and increase nutrient supply capacity in saline soil. These findings provide theoretical support for soil microbial improvement technology in the salinized field.

Comparative genomic analysis of Citrobacter sp. XT1-2-2 reveals insights into the molecular mechanism of microbial immobilization of heavy metals.

BACKGROUND: In our previous study, Citrobacter sp. XT1-2-2 was isolated from high cadmium-contaminated soils, and demonstrated an excellent ability to decrease the bioavailability of cadmium in the soil and inhibit cadmium uptake in rice. In addition, the strain XT1-2-2 could significantly promote rice growth and increase rice biomass. Therefore, the strain XT1-2-2 shows great potential for remediation of cadmium -contaminated soils. However, the genome sequence of this organism has not been reported so far.  RESULTS: Here the basic characteristics and genetic diversity of the strain XT1-2-2 were described, together with the draft genome and comparative genomic results. The strain XT1-2-2 is 5040459 bp long with an average G + C content of 52.09%, and contains a total of 4801 genes. Putative genomic islands were predicted in the genome of Citrobacter sp. XT1-2-2. All genes of a complete set of sulfate reduction pathway and various putative heavy metal resistance genes in the genome were identified and analyzed. CONCLUSIONS: These analytical results provide insights into the genomic basis of microbial immobilization of heavy metals.

Increased biomass and reduced tissue cadmium accumulation in rice via indigenous Citrobacter sp. XT1-2-2 and its mechanisms.

Microbial remediation is a promising technique to remediate heavy metals contaminated soils. In this study, the cadmium (Cd)- resistant Citrobacter sp. XT1-2-2, isolated from heavy metals contaminated paddy soils, was investigated to evaluate the effect of this strain on soil Cd speciation, cellular Cd distribution, tissue Cd accumulation and rice biomass. The percentage of Cd2+ removal by Citrobacter sp. XT1-2-2 was up to 82.3 ± 2.1% within 240 min in the solution. The average content of soil soluble plus exchangeable and carbonate-bound fractions of Cd decreased, whereas Fe/Mn oxide-bound, organic matter-bound and residual fractions increased with bacteria inoculation. For the paddy soil inoculated with the XT1-2-2 strain, Cd concentrations of roots, culms, leaves and grains were significantly reduced by 24.1%, 46.9%, 41.5% and 66.7%, respectively. In addition, inoculation bacteria significantly increased the biomass of the roots, above-ground tissues and the rice grains. All results indicated that the XT1-2-2 strain had the ability to immobilize soil Cd and decrease Cd accumulation in rice grains. Therefore, the XT1-2-2 strain has potential for application to remediate Cd-contaminated paddy soils. It is possible to exploit a new bacterial-assisted technique for the remediation in Cd-contaminated paddy soils.

Impacts of a Compound Amendment on Cd Immobilization, Enzyme Activities and Crop Uptake in Acidic Cd-Contaminated Paddy Soils.

This study assessed the effectiveness of limestone-montmorillonite-rapeseed residue-Si fertilizer compound amendment on the bioavailability and crop uptake of cadmium (Cd) and enzyme activities in acidic paddy soils. Applying the compound amendment at ratios of 1%-3% increased soil pH by 0.1-1.9 units, decreased leaching ratios of soil Cd 4.0%-22%, and decreased exchangeable and carbonated Cd 42%-55% and 27%-49%, respectively. Organic matter-bound Cd increased 47%-62% (p < 0.05). Cadmium concentrations decreased in the roots, culms, leaves, and grains of rice grown in the Cd-contaminated soils by 37%-81%, 18%-73%, 29%-64% and 27%-63%, respectively, (p < 0.05). Catalase and urease activities increased 2.5%-63% and 3.9%-36%, (p < 0.05), respectively. Applying this compound amendment may significantly mitigate soil acidification and decrease the bioavailability and crop uptake of Cd in acidic Cd-contaminated paddy soils.